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[86] studied a model of chronic cerebral hypoperfusion in adult rats caused by permanent ligation of both common carotid arteries

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As structure prediction models like AlphaFold increasingly integrate with humanization algorithms, future pipelines may directly generate clinical-grade, humanized nanobodies from target epitopes, significantly accelerating antibody drug discovery ( Although these computational tools mark a clear advance over traditional manual grafting, their validation approaches still suffer from notable limitations